Title
Chemical profiling, biostatic and biocidal dynamics of Origanum vulgare Lessential oil
Document Type
Article
Abstract
Origanum vulgare L. (Lamiaceae) is a widespread flavoring culinary and medicinal herb. The present study aimed at investigating the antimicrobial activity of Origanum vulgare (OV) essential oil (EO) through illustrating its biostatic, biocidal and the dynamics of the biocidal activity against 11 different microorganisms. GC/MS of OV EO allowed the identification of 32 compounds representing 99.94% of the oil. The two major identified compounds were terpinen-4-ol (38.35%) and trans-sabinene hydrate (10.06%). Different methods were employed to illustrate the biostatic activity of OV EO. Results of the biostatic studies on OV EO using agar and broth dilution methods showed that Staphylococcus aureus (S. aureus) was the most sensitive organism; with a Minimum inhibitor concentration (MIC) 1.18 mg/ml. Agar diffusion method showed that the highest activity was observed against Bordetella bronchiseptica (Br. bronchiseptica), Saccharomyces cerevisiae (S. cerevisiae), Bacillus subtilis (B. subtilus) and Staphylococcus epidermidis (S. epidermidis) with inhibition zones 38 ± 1.5, 29.5 ± 0.8, 26.9 ± 0.9 and 26.9 ± 1.1 mm, respectively. Studying the dynamics of 1% v/v OV essential oil emulsion over a period of 6 h revealed that Escherichia coli (E. coli), B. subtilis, S. epidermidis and S. cerevisiae had the fastest response. Also increasing concentrations of OV oil emulsion increased the rate of cell killing and the duration of growth lag phase increased correspondingly. These data indicated that OV EO produces a concentration and time-dependent antimicrobial activity.
Publication Date
12-1-2019
Faculty
Faculty of Applied Health Sciences Technology
Subject Area
Life Sciences, Biochemistry, Genetics, Molecular Biology
Indexed in Scopus
yes
DOI
https://doi.org/10.1186/s13568-019-0764-y
Volume
9
Keywords
Biocidal dynamics, Biostatic, Essential oil, GC/MS, Origanum vulgare, Terpinen-4-ol
eISSN
21910855
Recommended Citation
Fikry, Sahar; Khalil, Noha; and Salama, Osama, "Chemical profiling, biostatic and biocidal dynamics of Origanum vulgare Lessential oil" (2019). Faculty of Applied Health Sciences Technology. 159.
https://pks.pua.edu.eg/applied_health_publications/159