Title

Intestinal schistosomiasis among preschool and school-aged children in a rural setting near Alexandria: initiative for elimination

Document Type

Article

Abstract

Objective: To assess the status of intestinal schistosomiasis among preschool-aged (PSAC) and school-aged children (SAC) and to compare the efficacy of praziquantel (PZQ) in both groups. Methods: The study was conducted on 400 children; 103 PSAC and 297 SAC. Diagnosis of Schistosoma mansoni was based on triplicate Kato-Katz thick smears from a single stool sample. To identify the missed cases by Kato-Katz, 120 randomly selected negative cases (38 PSAC and 82 SAC) were screened by real-time PCR. All S. mansoni-positive cases by Kato-Katz were treated by crushed PZQ tablets. Four weeks after treatment, the cure rate was assessed by Kato-Katz smears and real-time PCR. Results: The prevalence of S. mansoni with Kato-Katz was 7.8% among PSAC and 7.4% among SAC. Most of children (63.3%) had light-intensity infection. The cure rate was 100% among PSAC by both techniques, and 91%, and 77.2% among SAC by Kato-Katz and real-time PCR, respectively. In the 120 stool samples screened by real-time PCR, S. mansoni prevalence was 25%; 15.8% and 29.3% were among PSAC and SAC respectively. Treated cases showed a lower range of Ct values than untreated cases. Two melting temperature ranges (Tm = 83–87°C and 89–93°C) were recognised among uncured cases which may point to S. mansoni genetic variability. Conclusion: Continuous monitoring and inclusion of PSAC in schistosomiasis control programmes are crucial. Real-time PCR and other molecular tools are recommended for evaluation of the true prevalence, assessment of cure and further studies on genetic diversity.

Publication Date

6-1-2021

Faculty

Faculty of Applied Health Sciences Technology

Subject Area

Life Sciences, General Immunology and Microbiology, Health Sciences, General Medicine

Indexed in Scopus

yes

Indexed in Web Of Science

yes

DOI

https://doi.org/10.1111/tmi.13562

Volume

26

Keywords

Egypt, Kato-Katz, praziquantel, preschool-aged children, real-time PCR, Schistosoma mansoni, school-aged children

ISSN

13602276

eISSN

13653156

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