Title

Recombinant amelogenin protein induces apical closure and pulp regeneration in open-apex, nonvital permanent canine teeth

Document Type

Article

Abstract

Introduction Recombinant DNA-produced amelogenin protein was compared with calcium hydroxide in a study of immature apex closure conducted in 24 young mongrel dogs. Methods Root canals of maxillary and mandibular right premolars (n = 240) were instrumented and left open for 14 days. Canals were cleansed, irrigated, and split equally for treatment with recombinant mouse amelogenin (n = 120) or calcium hydroxide (n = 120). Results After 1, 3, and 6 months, the animals were sacrificed and the treated teeth recovered for histologic assessment and immunodetection of protein markers associated with odontogenic cells. After 1 month, amelogenin-treated canals revealed calcified tissue formed at the apical foramen and a pulp chamber containing soft connective tissue and hard tissue; amelogenin-treated canals assessed after 3- and 6-month intervals further included apical tissue functionally attached to bone by a periodontal ligament. In contrast, calcified apical tissue was poorly formed in the calcium hydroxide group, and soft connective tissue within the pulp chamber was not observed. Conclusions The findings from this experimental strategy suggest recombinant amelogenin protein can signal cells to enhance apex formation in nonvital immature teeth and promote soft connective tissue regeneration.

Publication Date

3-1-2016

Faculty

Faculty of Dentistry

Subject Area

Health Sciences, General Dentistry

Indexed in Scopus

yes

Indexed in Web Of Science

yes

DOI

https://doi.org/10.1016/j.joen.2015.11.003

Volume

42

Keywords

Amelogenin, apex formation, calcium hydroxide, pulp regeneration, regeneration

ISSN

00992399

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