Title

Kinetic studies on the expression of alginate and extracellular proteins by pseudomonas aeruginosa frd1 and pao1

Document Type

Article

Abstract

Pseudomonas aeruginosa is characterized by its capability to produce extracellular virulence proteins and to establish biofilm-based infections that do not respond easily to conventional treatments. How-ever, the physiological conditions that decrease the fitness of such a persistent pathogen would assist the host to defend itself and reduce the infection prevalence. Therefore, developing treatments against P. aeruginosa requires a quantitative understanding of the relationship between bacterial growth kinetics and secretion of alginate and pro-teins, in addition to the ecological factors that control their synthesis. For this purpose, we examined various environmental factors that affect the specific product yield coefficients (expressed as g prod-uct/OD600) of alginate and extracellular proteins using a mucoid (FRD1) and a non-mucoid (PAO1) clinical isolate of P. aeruginosa, respectively. The results suggested magnesium sulfate, trace elements and hydrogen peroxide as significant variables that positively affect alginate synthesis by the FRD1 cells. However, the production of extracellular proteins by PAO1 was negatively affected by the con-centration of ferrous sulfate. For understanding the kinetics of expressing alginate and extracellular proteins by the cells, a well-controlled 5 L tank bioreactor was used. The results suggested that under the bioreactor controlled conditions, both alginate and extracellular proteins are expressed parallel to biomass increase in the cells of P. aeruginosa.

Publication Date

1-1-2020

Faculty

Faculty of Dentistry

Subject Area

Life Sciences, General Immunology and Microbiology

Indexed in Scopus

yes

Indexed in Web Of Science

yes

DOI

https://doi.org/10.2323/jgam.2019.04.003

Volume

66

Keywords

Alginate, Extracellular polymeric substances (EPS), Extracellular proteins, Kinetics, Polysaccharides, Pseu-domonas aeruginosa

ISSN

00221260

eISSN

13498037

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