A novel HPLC-DAD method for simultaneous determination of febuxostat and diclofenac in biological samples: Pharmacokinetic outcomes
Aim: To develop a simple HPLC-DAD method for simultaneous determination of febuxostat (FEB) and diclofenac (DIC) in biological samples to assess pharmacokinetic outcomes of their coadministration. Methodology & results: Sample preparation was performed by liquid-liquid extraction. Drugs analysis was done on C18 column using methanol-formic acid pH 2.1 (76:24, v/v) as mobile phase and time-programmed UV detection. Lower limits of quantitation for FEB and DIC were 10 and 20 ng/ml, respectively. Baseline pharmacokinetics were similar to published data on either drug alone. Coadministration led to more than twofold increase in FEB C max and AUC together with a reduced hepatic uptake in rats. Conclusion: DIC interfered with initial distribution and terminal clearance of FEB potentially due to reduced FEB hepatic uptake.
Faculty of Pharmacy
Health Sciences, General Health Professions, Life Sciences, Pharmacology, Toxicology, Pharmaceutical Science, Biochemistry, Genetics, Molecular Biology, Physical Sciences, General Chemistry
Indexed in Scopus
Indexed in Web Of Science
diclofenac, febuxostat, HPLC-DAD, human plasma, liver uptake, pharmacokinetics
El-Yazbi, Fawzy A.; Amin, Omayma A.; El-Kimary, Eman I.; Khamis, Essam F.; Younis, Sameh E.; Elkhatib, Mohammed A.W.; and El-Yazbi, Ahmed F., "A novel HPLC-DAD method for simultaneous determination of febuxostat and diclofenac in biological samples: Pharmacokinetic outcomes" (2019). Faculty of Pharmacy. 25.