Title

A novel HPLC-DAD method for simultaneous determination of febuxostat and diclofenac in biological samples: Pharmacokinetic outcomes

Document Type

Article

Abstract

Aim: To develop a simple HPLC-DAD method for simultaneous determination of febuxostat (FEB) and diclofenac (DIC) in biological samples to assess pharmacokinetic outcomes of their coadministration. Methodology & results: Sample preparation was performed by liquid-liquid extraction. Drugs analysis was done on C18 column using methanol-formic acid pH 2.1 (76:24, v/v) as mobile phase and time-programmed UV detection. Lower limits of quantitation for FEB and DIC were 10 and 20 ng/ml, respectively. Baseline pharmacokinetics were similar to published data on either drug alone. Coadministration led to more than twofold increase in FEB C max and AUC together with a reduced hepatic uptake in rats. Conclusion: DIC interfered with initial distribution and terminal clearance of FEB potentially due to reduced FEB hepatic uptake.

Publication Date

1-1-2019

Faculty

Faculty of Pharmacy

Subject Area

Health Sciences, General Health Professions, Life Sciences, Pharmacology, Toxicology, Pharmaceutical Science, Biochemistry, Genetics, Molecular Biology, Physical Sciences, General Chemistry

Indexed in Scopus

yes

Indexed in Web Of Science

yes

DOI

https://doi.org/10.4155/bio-2018-0219

Volume

11

Keywords

diclofenac, febuxostat, HPLC-DAD, human plasma, liver uptake, pharmacokinetics

ISSN

17576180

eISSN

17576199

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